• Product NameTOMM20 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total TOMM20 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human TOMM20.
• Target NameTOMM20
• Alternative Names    MAS20; MOM19; TOM20;
• Accession No. Swiss-Prot#: Q15388
  NCBI Gene ID: 9804
• UniprotQ15388
• Gene ID9804;
• SDS-PAGE MW 16kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:20 - 1:50

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity)

• Western blot analysis of extracts of various cell lines, using TOM20 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded human colon using TOM20 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human colon carcinoma using TOM20 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human liver cancer using TOM20 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human lung cancer using TOM20 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded rat ovary using TOM20 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded rat heart using TOM20 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse heart using TOM20 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of HeLa cells using TOM20 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting