• Product NamePOLD3 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total POLD3 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human POLD3 (NP_006582.1).
• Target NamePOLD3
• Alternative Names    POLD3;P66;P68;PPP1R128
• Accession No. Uniprot:Q15054
  GeneID:10714
• UniprotQ15054
• Gene ID10714
• SDS-PAGE MW 51kDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IF 1:50 - 1:100

This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene.

• Western blot analysis of extracts of various cell lines, using POLD3 antibody.
• Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLD3 antibody.
• Immunofluorescence analysis of U2OS cells using POLD3 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting