• Product NameHistone H3K27AC antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total Histone H3K27AC protein.
• Immunogen TypePeptide
• Immunogen DescriptionA synthetic acetylated peptide corresponding to residues surrounding K27 of human Histone H3.
• Target NameHistone
• Alternative Names    H3t; H3.4; H3/g; H3FT;
• Accession No. Swiss-Prot#: Q16695
  NCBI Gene ID: 8290
• UniprotQ16695
• Gene ID8290;
• SDS-PAGE MW 16kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200
  IP 1:50 - 1:200
  ChIP 1:20 - 1:100
  ChIPseq 1:20 - 1:100

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

• Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A17559) at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A17559) at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A17559) at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K27 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K27 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K27 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K27 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded human breast using Acetyl-Histone H3-K27 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K27 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded rat heart using Acetyl-Histone H3-K27 at dilution of 1:200 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting