• Product NamePOU6F1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total POU6F1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human POU6F1.
• Target NamePOU6F1
• Alternative Names    BRN5; MPOU; TCFB1;
• Accession No. Swiss-Prot#: Q14863
  NCBI Gene ID: 5463
• UniprotQ14863
• Gene ID5463;
• SDS-PAGE MW 33kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:100

Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3').

• Immunofluorescence analysis of A-549 cells using POU6F1 .
• Western blot analysis of extracts of various cell lines, using POU6F1 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting