• Product NameFBXL3 (Phospho-Ser320) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of FBXL3 only when phosphorylated at serine 320.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 320 (S-V-S(p)-K-D) derived from Human FBXL3.
• Target NameFBXL3
• ModificationPhospho
• Alternative Names    FBL3A; FBXL3A
• Accession No. Swiss-Prot#: Q9UKT7
  NCBI Protein#: NP_036290.1.
• UniprotQ9UKT7
• Gene ID26224;
• SDS-PAGE MW 49kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C

• Western blotting: 1:500~1:1000

 Substrate-recognition component of the SCF(FBXL3) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation. The SCF(FBXL3) complex mainly acts in the nucleus and mediates ubiquitination and subsequent degradation of CRY1 and CRY2. Activity of the SCF(FBXL3) complex is counteracted by the SCF(FBXL21) complex.

• Western blot analysis of extracts from Hela cells treated with TNFa (lane 2) and mouse brain tissus(lane 1) using FBXL3 (Phospho-Ser320) Antibody #11593.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting