• Product Namep53(Phospho-Ser37) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of p53 only when phosphorylated at serine 37.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 37 (L-P-S(p)-Q-A) derived from Human p53.
• Target Namep53
• ModificationPhospho
• Alternative Names    Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53;
• Accession No. Swiss-Prot: P04637
  NCBI Protein: NP_000537.3
• UniprotP04637
• Gene ID7157;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 53kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

p53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes

Ito, A. et al. (2001) EMBO J. 20, 1331-1340.
Sakaguchi, K. et al. (1998) Genes Dev. 12, 2831-2841.
Solomon, J.M. et al. (2006) Mol. Cell. Biol. 26, 28-38.

• Immunofluorescence staining of methanol-fixed Hela cells using p53(Phospho-Ser37) Antibody #11098.
• Western blot analysis of extracts from HT29 cells untreated or treated with UV, and MCF cells untreated or treated with Anisomycin using p53 (Phospho-Ser37) Antibody #11098.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting