• Product Namep53(Phospho-Ser315) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of p53 only when phosphorylated at serine 315.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 315 (S-S-S(p)-P-Q) derived from Human p53.
• Target Namep53
• ModificationPhospho
• Alternative Names    Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53;
• Accession No. Swiss-Prot: P04637
  NCBI Protein: NP_000537.3
• UniprotP04637
• Gene ID7157;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 53kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.
Lohrum, M. et.al. (1996) Oncogene 13, 2527-2539.
Posp

• Western blot analysis of extracts from Hela cells using p53(Phospho-Ser315) Antibody #11100(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53(Phospho-Ser315) Antibody #11100(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using p53(Phospho-Ser315) Antibody #11100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting