• Product NameJAM-B antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 264 of Human JAM2
• Target NameJAM-B
• Accession No. Swiss-Prot:P57087
  Gene ID:58494
• UniprotP57087
• Gene ID58494;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 33kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250
  

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: A431
  12 % SDS PAGE
  Primary antibody diluted at 1: 1000
• A:Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using JAM-B antibody at 1: 100 dilution. B:Immunohistochemical analysis of paraffin-embedded human colon, using JAM-B antibody at 1: 200 dilution. C:Immunohistochemical analysis of paraffin-embedded mouse small intestine, using JAM-B antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting