• Product NameDopamine beta-Hydroxylase antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 8 and 280 (P09172) of Dopamine beta hydroxylase
• Target NameDopamine beta-Hydroxylase
• Accession No. Swiss-Prot:P09172
  Gene ID:1621
• UniprotP09172
• Gene ID1621;
• Concentration 0.22 mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 69kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250
  Immunofluorescence: 1:100-1:200

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: Hep G2
  7.5% SDS PAGE
  Primary antibody diluted at 1: 5000
• Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Dopamine beta-Hydroxylase antibody at 1: 500 dilution.
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Dopamine beta-Hydroxylase antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting