• Product NameMAPK12 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MAPK12 protein.
• Immunogen TypeProtein
• Immunogen DescriptionFull length fusion protein
• Target NameMAPK12
• Alternative Names    ERK3; ERK6; SAPK3; PRKM12; SAPK-3; P38GAMMA
• Accession No. Swiss-Prot:P53778
  Gene Accssion:BC015741
• UniprotP53778
• Gene ID6300;
• SDS-PAGE MW 42KD
• Concentration 1.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry:1:25-1:100

Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes.?

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Mouse muscle tissue and Jurkat cell, K562 and A431 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/350 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #40092 at dilution 1/25.
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #40092 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting