• Product NameTMED4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total TMED4 protein.
• Immunogen TypeProtein
• Immunogen DescriptionFusion protein of human transmembrane emp24 protein transport domain containing 4
• Target NameTMED4
• Alternative Names    HNLF; ERS25; GMP25iso; p24alpha3
• Accession No. Swiss-Prot:Q7Z7H5
  Gene Accssion:BC057851
• UniprotQ7Z7H5
• Gene ID222068;
• SDS-PAGE MW 26KD
• Concentration 2.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:5000
  Immunohistochemistry:1:30-1:150

Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis (By similarity).

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Hepg2, K562 and hela cell, A549 cell and mouse intestines tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/800 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #40156 at dilution 1/45.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #40156 at dilution 1/45.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting