• Product NameHCRT Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total HCRT protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human hypocretin (orexin) neuropeptide precursor
• Target NameHCRT
• Alternative Names    OX; PPOX; NRCLP1
• Accession No. Swiss-Prot:O43612
  Gene Accssion:NP_001515
• UniprotO43612
• Gene ID3060;
• SDS-PAGE MW 13KD
• Concentration 1.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.

• Gel: 12%SDS-PAGE
  Lysate: 40ug Mouse muscle tissuePrimary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 40 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting