• Product NameTPST1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total TPST1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide of human tyrosylprotein sulfotransferase 1
• Target NameTPST1
• Alternative Names    TANGO13A
• Accession No. Swiss-Prot:O60507
  Gene Accssion:NP_003587
• UniprotO60507
• Gene ID8460;
• SDS-PAGE MW 42KD
• Concentration 1.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000

The tyrosylprotein sulfotransferases TPST-1 and TPST-2 catalyze the sulfation of tyrosine residues within secreted and membrane-bound proteins, such as cell adhesion molecules, G-protein-coupled receptors, coagulation factors, serpins, extracellular matrix proteins, and hormones. Although both TPST-1 and TPST-2 utilize 3¡¯-phosphoadenosine 5¡¯-phosphosulfate as their sulfate donor, they differ in their substrate specificity.?

• Gel: 8%SDS-PAGE
  Lysates (from left to right): 231, 293T and lovo cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting