• Product NameTRIM45 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total TRIM45 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the N terminal of human tripartite motif containing 45
• Target NameTRIM45
• Alternative Names    RNF99
• Accession No. Swiss-Prot:Q9H8W5
  Gene Accssion:NP_079464
• UniprotQ9H8W5
• Gene ID80263;
• SDS-PAGE MW 64KD
• Concentration 0.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:100-1:200

This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): 231, Jurkat, A549, hela and 293T cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human Colorectal cancer tissue using #40382 at dilution 1/100.
• Immunohistochemical analysis of paraffin-embedded Human Colorectal cancer tissue using #40382 at dilution 1/200.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting