• Product NameTOP2B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total TOP2B protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human topoisomerase (DNA) II beta 180kDa
• Target NameTOP2B
• Alternative Names    TOPIIB; top2beta
• Accession No. Swiss-Prot:Q02880
  Gene Accssion:NP_001059
• UniprotQ02880
• Gene ID7155;
• SDS-PAGE MW 183KD
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.?

• Gel: 6%SDS-PAGE
  Lysates (from left to right): Raw264.7, Jurkat, K562 and hepg2 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/250 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 40 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting