• Product NameTRIM47 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total TRIM47 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human tripartite motif containing 47
• Target NameTRIM47
• Alternative Names    GOA; RNF100
• Accession No. Swiss-Prot:Q96LD4
  Gene Accssion:NP_258411
• UniprotQ96LD4
• Gene ID91107;
• SDS-PAGE MW 70KD
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry:1:25-1:100

TRIM47 (tripartite motif-containing 47), also known as GOA (Gene overexpressed in astrocytoma protein) or RNF100 (RING finger protein 100), is a 638 amino acid protein that localizes to both cytoplasm and nucleus and belongs to the TRIM/RBCC family. While TRIM47 expression is low in most tissues, it is highly expressed in kidney tubular cells and overexpressed in astrocytoma tumor cells.?

• Gel: 8%SDS-PAGE
  Lysate: 40ug 293T cellPrimary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue using #40392 at dilution 1/30.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #40392 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting