• Product NameRecombinant Human Phospholipase A2, membrane associated protein(PLA2G2A)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• PurificationGreater than 90% as determined by SDS-PAGE.
• Immunogen DescriptionExpression Region:21-144aa
  Sequence Info:Full Length
• Alternative Names    GIIC sPLA2Group IIA phospholipase A2Non-pancreatic secretory phospholipase A2 ;NPS-PLA2;Phosphatidylcholine 2-acylhydrolase 2A
• Accession No. P14555
• UniprotP14555
• Gene ID5320;
• Calculated MW 17.9 kDa
• Tag Info N-terminal 6xHis-tagged
• Target Sequence NLVNFHRMIKLTTGKEAALSYGFYGCHCGVGGRGSPKDATDRCCVTHDCCYKRLEKRGCGTKFLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKYQYYSNKHCRGSTPRC
• Formulation Tris-based buffer50% glycerol
• Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
  Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

Thought to participate in the regulation of the phospholipid metabolism in biombranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Cloning and recombinant expression of phospholipase A2 present in rheumatoid arthritic synovial fluid.Seilhamer J.J., Pruzanski W., Vadas P., Plant S., Miller J.A., Kloss J., Johnson L.K.J. Biol. Chem. 264:5335-5338(1989)
Research Topic:Metabolism

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting