• Product NamePyruvate Kinase(liver/RBC) antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 230 of Pyruvate Kinase(liver/RBC)
• Target NamePyruvate Kinase(liver/RBC)
• Accession No. Swiss-Prot:P30613
  Gene ID:5313
• UniprotP30613
• Gene ID5313;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 62kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:50-1:500
  Immunofluorescence: 1:100-1:200
  

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: H1299
  B: Hela
  7.5% SDS PAGE
  Primary antibody diluted at 1: 5000
• Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase (liver/RBC) antibody at 1: 500 dilution.
• Immunofluorescence analysis of paraformaldehyde-fixed A431, using Pyruvate Kinase(liver/RBC) antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting