• Product NameZO-1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC
• Species ReactivityHu
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 266 of Human TJP1
• Target NameZO-1
• Accession No. Swiss-Prot:Q07157
  Gene ID:7082
• UniprotQ07157
• Gene ID7082;
• Concentration 0.22 mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 187kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

• Sample(30 ug whole cell lysate)
  A: H1299
  5% SDS PAGE
  Primary antibody diluted at 1: 3000
• Immunohistochemical analysis of paraffin-embedded H661 xenograft, using ZO-1 antibody at 1: 250 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting