ASC Polyclonal Antibody - SAB | Signalway Antibody

Catalog No.	Package Size	Price	Quantity
40618-1	50ul	$219.00
40618-2	100ul	$319.00

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Description

Product NameASC Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB IHC IF
Species ReactivityHu Ms Rt
SpecificityASC Polyclonal Antibody detects endogenous levels of ASC protein.
Immunogen DescriptionSynthesized peptide derived from the N-terminal region of human ASC.
Target NameASC
Alternative Names PYCARD; ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; hASC; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1

Accession No. Swiss-Prot: Q9ULZ3
NCBI Gene ID: 29108
UniprotQ9ULZ3
Gene ID29108;
SDS-PAGE MW 21kd
Concentration 1mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Store at -20˚C/1 year

Application Details

Images

Western Blot analysis of 823 293T cells using ASC Polyclonal Antibody
Wu, Dong-Dong, et al. "Inhibition of alveolar macrophage pyroptosis reduces lipopolysaccharide-induced acute lung injury in mice." Chinese medical journal 128.19 (2015): 2638.
Western blot analysis of lysates from 1) K562 , 2) Hela , 3) 3T3 cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. （Red） Tubulin β Monoclonal Antibody(5G3) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody was diluted at 1:10000, 37° 1hour.
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,ASC Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,ASC Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using ASC Antibody. The picture on the right is blocked with the synthesized peptide.

Published Papers

The Extracts of Angelica sinensis and Cinnamomum cassia from Oriental Medicinal Foods Regulate Inflammatory and Autophagic Pathways against Neural Injury after Ischemic Stroke. In Oxid Med Cell Longev on 2021 Jun 26 by Cheng Luo, Qi Chen,et al..PMID:34257822, , (2021),
PMID: 34257822 Alpha-lipoic acid alleviates NAFLD and triglyceride accumulation in liver via modulating hepatic NLRP3 inflammasome activation pathway in type 2 diabetic rats. In Food Sci Nutr on 2021 Mar 13 by Chih-Yuan Ko, Yangming Martin Lo, et al..PMID: 34026086, , (2021),
PMID: 34026086 Protective effect of ginsenoside Rg5 against kidney injury via inhibition of NLRP3 inflammasome activation and the MAPK signaling pathway in high-fat diet/streptozotocin-induced diabetic mice. In Pharmacol Res on 2020 May by Yanyan Zhu, Chenhui Zhu, et al..PMID: 32156650, , (2020),
PMID: 32156650 Ginsenoside Rk3 alleviated DSS-induced ulcerative colitis by protecting colon barrier and inhibiting NLRP3 inflammasome pathway. In Int Immunopharmacol on 2020 Aug by Mi Tian, Pei Ma, et al..PMID: 32521491, , (2020),
PMID: 32521491 Please let us know if you have published research using #40618 so that we can cite your reference.

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ASC Polyclonal Antibody #40618

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Species Reactivity