• Product NameGrowth/differentiation factor 5
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• PurificationGreater than 90% by SDS-PAGE
• Species ReactivityHuman
• Immunogen Description28-381AA
• Alternative Names    BMP14,CDMP1,Bone morphogenetic protein 14,Cartilage-derived morphogenetic protein 1,Lipopolysaccharide-associated protein 4,Radotermin,BMP-14,CDMP-1,LAP-4
• Accession No. P43026
  Gene name:GDF5
• UniprotP43026
• Gene ID8200;
• Calculated MW 38.94
• Tag Info His
• Formulation 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole,10%glycerol(PH8.0)
• Storage Store at -20C. (Avoid repeated freezing and thawing.)
  Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (PubMed:24098149, PubMed:21976273, PubMed:15530414, PubMed:25092592). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (PubMed:21976273). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (By similarity). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205).

Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting