• Product NameEEF2K antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 238 of EEF2K
• Target NameEEF2K
• Accession No. Swiss-Prot:O00418
  Gene ID:29904
• UniprotO00418
• Gene ID29904;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 82kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:50-1:500
  Immunofluorescence: 1:100-1:200
  

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: A431
  B: H1299
  C: Hela
  D: Hep G2
  7.5% SDS PAGE
  Primary antibody diluted at 1: 5000
• Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EEF2K antibody at 1: 500 dilution.
• Immunofluorescence analysis of paraformaldehyde-fixed A431, using EEF2K antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting