• Product NameAmiloride hydrochloride dihydrate
• Brief DescriptionInhibitors
• Purification99.50%
• Biological ActivityA pyrazine compound inhibiting SODIUM reabsorption through SODIUM CHANNELS in renal EPITHELIAL CELLS. This inhibition creates a negative potential in the luminal membranes of principal cells, located in the distal convoluted tubule and collecting duct. Negative potential reduces secretion of potassium and hydrogen ions. Amiloride is used in conjunction with DIURETICS to spare POTASSIUM loss. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p705)
• Target NameSodium Channel inhibitor
• CAS No. 17440-83-4
• Calculated MW 302.12
• Formulation C6H8ClN7OHCl2H2O
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Other
  SMILES:O.O.Cl.NC(N)=NC(=O)C1=C(N)N=C(N)C(Cl)=N1
  Pathways:Membrane transporter/Ion channel
  Receptor:Amiloride-sensitive sodium channel
  Boling pt:628.1C
  Melting pt:
  Solubility:DMSO: 60 mg/mL (198.59 mM)
  Appearance:Faint Yellow Powder
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Kelly O, et al. Am J Physiol Renal Physiol. 2003 Dec;285(6):F1279-90.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting