• Product NamePyrimethamine
• Brief DescriptionInhibitors
• Purification99.74%
• Biological ActivityPyrimethamine is a dihydrofolate reductase(DHFR) inhibitor with an IC50 of 15.4 nM. It is a medication used for protozoal infections. It is commonly used as an antimalarial drug (for both treatment and prevention of malaria), and is also used (combined with sulfadiazine) in the treatment of Toxoplasma gondii infections in immune-compromised patients, such as HIV-positive individuals. It interferes with folic acid synthesis by inhibiting the enzyme dihydrofolate reductase (DHFR). Folic acid is needed for DNA and RNA synthesis in many species, including protozoa.
• Target NameDHFR inhibitor
• CAS No. 58-14-0
• Calculated MW 248.71
• Formulation C12H13ClN4
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Cancer
  SMILES:CCC1=C(C(N)=NC(N)=N1)C1=CC=C(Cl)C=C1
  Pathways:Metabolism
  Receptor:DHFR
  Boling pt:
  Melting pt:233-234C
  Solubility:DMSO: 10 mg/mL (40.2 mM)
  Appearance:White Powder
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Rastelli G, et al. Bioorg Med Chem Lett. 2003 Oct 6;13(19):3257-60.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting