• Product NameMethotrexate
• Brief DescriptionInhibitors
• Purification97.43%
• Biological ActivityMethotrexate, a folate antagonist, is a potent anti-inflammatory agent when used weekly in low concentrations, the anti-phlogistic action of which is due to increased adenosine release at inflamed sites. Studies have demonstrated that methotrexate polyglutamates are active inhibitors of several enzymatic reactions, including dihydrofolate reductase.
• Target NameDehydrogenase inhibitor
• CAS No. 1959-5-2
• Calculated MW 454.44
• Formulation C20H22N8O5
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Cancer
  SMILES:CN(CC1=CN=C2C(=N1)C(=NC(=N2)N)N)C3=CC=C(C=C3)C(=O)N[C@@H](CCC(=O)O)C(=O)O
  Pathways:Metabolism
  Receptor:hDHFR
  Boling pt:
  Melting pt:195C
  Solubility:DMSO: 90 mg/mL warmed (198.04 mM)
  Appearance:Yellow Powder
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Al-Rashood ST, et al. Bioorg Med Chem. 2006 Dec 15;14(24):8608-21.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting