• Product NameAfatinib (BIBW2992) Dimaleate
• Brief DescriptionInhibitors
• Purification99.66%
• Biological ActivityAfatinib (BIBW2992) irreversibly inhibits EGFR/HER2 including EGFR(wt), EGFR(L858R), EGFR(L858R/T790M) and HER2 with IC50 of 0.5 nM, 0.4 nM, 10 nM and 14 nM, respectively; 100-fold more active against Gefitinib-resistant L858R-T790M EGFR mutant.
• Target NameEGFR inhibitor; HER inhibitor
• CAS No. 850140-73-7
• Calculated MW 718.08
• Formulation C32H33ClFN5O11
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Cancer
  SMILES:CN(C/C=C/C(=O)NC1=C(C=C2N=CN=C(C2=C1)NC3=CC(=C(C=C3)F)Cl)O[C@@H]4COCC4)C.C(=C\C(=O)O)\C(=O)O.C(=C\C(=O)O)\C(=O)O
  Pathways:Angiogenesis; JAK/STAT signaling; Tyrosine Kinase/Adaptors
  Receptor:EGFR (L858R); EGFR (L858R/T790M); EGFR (wt); HER2
  Boling pt:
  Melting pt:
  Solubility:DMSO: 100 mg/mL (139.43 mM)
  Appearance:White Powder
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. D Li, et al. Oncogene (2008) 27, 4702-4711

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting