• Product NamePurmorphamine
• Brief DescriptionInhibitors
• Purification98.00%
• Biological ActivityPurmorphamine, which directly binds and activates Smoothened, blocks BODIPY-cyclopamine binding to Smo with IC50 of ~ 1.5 μM in HEK293T cell and also is an inducer of osteoblast differentiation with EC50 of 1 μM.
• Target NameHedgehog/Smoothened antagonist
• CAS No. 483367-10-8
• Calculated MW 520.62
• Formulation C31H32N6O2
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Other
  SMILES:C1CCC(CC1)N1C=NC2=C1N=C(OC1=CC=CC3=CC=CC=C13)N=C2NC1=CC=C(C=C1)N1CCOCC1
  Pathways:GPCR/G Protein; Stem Cells
  Receptor:Smoothened
  Boling pt:
  Melting pt:
  Solubility:DMSO: 4 mg/mL warmed (7.68 mM)
  Appearance:Faint Green Powder
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Faghihi F, Biomed PharmacOthers, 2012, 3322(12).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting