• Product NameD 4476
• Brief DescriptionInhibitors
• Purification99.80%
• Biological ActivityD 4476 is a potent, selective, and cell-permeant CK1 (casein kinase 1) inhibitor with IC50 of 200 nM and 300 nM in a cell-free assay for CK1 from Schizosaccharomyces pombe and CK1δ, respectively. Also acts as an ALK5 inhibitor with IC50 of 500 nM.
• Target NameALK inhibitor; Casein Kinase inhibitor
• CAS No. 301836-43-1
• Calculated MW 398.41
• Formulation C23H18N4O3
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Metabolism system
  SMILES:NC(=O)C1=CC=C(C=C1)C1=NC(=C(N1)C1=CC=CC=N1)C1=CC2=C(OCCO2)C=C1
  Pathways:Metabolism; Angiogenesis; Stem Cells; Tyrosine Kinase/Adaptors
  Receptor:ALK5; CK1; CK1δ
  Boling pt:
  Melting pt:
  Solubility:DMSO: 79 mg/mL (198.28 mM)
  Appearance:White Powder
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Rena G, et al. EMBO Rep. 2004, 5(1), 60-65.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting