• Product NameAG-337;Nolatrexed dihydrochloride
• Brief DescriptionInhibitors
• Purification99.50%
• Biological ActivityNolatrexed Dihydrochloride is the dihydrochloride salt of nolatrexed, a water-soluble lipophilic quinazoline folate analog with antineoplastic activity. Nolatrexed occupies the folate binding site of thymidylate synthase, resulting in inhibition of thymidylate synthase activity and thymine nucleotide synthesis with subsequent inhibition of DNA replication, DNA damage, S-phase cell cycle arrest, and caspase-dependent apoptosis. This agent also exhibits radiosensitizing activity.
• Target NameDNA/RNA Synthesis inhibitor
• CAS No. 152946-68-4
• Calculated MW 357.26
• Formulation C14H14Cl2N4OS
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Cancer
  SMILES:CC1=C(SC2=CC=NC=C2)C2=C(NC(N)=NC2=O)C=C1
  Pathways:DNA Damage/DNA Repair
  Receptor:Thymidylate synthase
  Boling pt:
  Melting pt:301-302C
  Solubility:DMSO: > 10 mM
  Appearance:White Powder
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Hughes AN, et al. Br J Y. 2000 May;82(9):1519-27.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting