• Product NameTimosaponin AIII
• Brief DescriptionInhibitors
• Purification95.00%
• Biological ActivityTimosaponin AIII induces autophagy in HeLa cells followed by apoptotic cell death (IC50=10μM). The Timosaponin AIII cellular response has been found to be mediated via inhibition of mTORC1 and induction of ER stress (IC50 for BT474 cells=2.5?M and MDAMB231 cells=6?M). The Timosaponin AIII pro-apoptotic response is selective for tumor cells over normal cells but autophagy is induced in both.
• Target NamemTOR inhibitor
• CAS No. 41059-79-4
• Calculated MW 740.92
• Formulation C39H64O13
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Others
  SMILES:C[C@H]1[C@H]2[C@H](C[C@@H]3[C@@]2(CC[C@H]4[C@H]3CC[C@H]5[C@@]4(CC[C@@H](C5)O[C@H]6[C@@H]([C@H]([C@H]([C@H](O6)CO)O)O)O[C@H]7[C@@H]([C@H]([C@@H]([C@H](O7)CO)O)O)O)C)C)O[C@]18CCC(CO8)C
  Pathways:PI3K/Akt/mTOR signaling
  Receptor:mTORC1
  Boling pt:
  Melting pt:
  Solubility:Soluble in Pyridine, Methanol, Ethanol, etc.
  Appearance:Powder
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Wu ZT, et al. Acta Pharmacol Sin. 2014 Sep;35(9):1188-98.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting