• Product NameRO8994
• Brief DescriptionInhibitors
• Purification98.00%
• Biological ActivityRO8994 is a highly potent and selective series of spiroindolinone small-molecule MDM2 inhibitor, with IC50 of 5 nM (HTRF binding assays) and 20 nM (MTT proliferation assays).
• Target NameMdm2 inhibitor; p53 inhibitor
• CAS No. 1309684-94-3
• Calculated MW 613.51
• Formulation C31H31Cl2FN4O4?
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Others
  SMILES:O=C([C@H](N[C@H]1CC(C)(C)C)[C@@H](C2=CC=CC(Cl)=C2F)[C@@]31C(NC4=C3C=CC(Cl)=C4)=O)NC5=CC=C(C(N)=O)C=C5OC
  Pathways:Apoptosis
  Receptor:Mdm2; P53
  Boling pt:
  Melting pt:
  Solubility:DMSO: 45 mg/mL
  Appearance:
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Zhang Z,et al. Discovery of potent and selective spiroindolinone MDM2 inhibitor, RO8994, for cancer therapy. Bioorg Med Chem. 2014 Aug 1;22(15):4001-9.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting