• Product NameGW 4869
• Brief DescriptionInhibitors
• Purification90.00%
• Biological ActivityA cell-permeable, symmetrical dihydroimidazolo-amide compound that acts as a potent, specific, non-competitive inhibitor of N-SMase (neutral sphingomyelinase) [IC50?= ~ 1 ?M, rat brain; Km?for sphingomyelin ~13 ?M]. Does not inhibit human A-SMase (acid sphingomyelinase) even at 150 ?M. Weakly inhibits the activities of bovine protein phosphatase 2A and mammalian lyso-PAF PLC, while no inhibition is observed for bacterial phosphatidylcholine-specific PLC. Reported to offer complete protection against TNF-α or diamine-induced cell death in MCF7 breast Y cells at 20 ?M. Does not modify the intracellular glutathione levels or interfere with TNF-α or diamine-mediated signaling effects.
• Target NamePhospholipase inhibitor
• CAS No. 6823-69-4
• Calculated MW 577.5
• Formulation C30H30Cl2N6O2
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Others
  SMILES:C1NC(=NC1)C2=CC=C(C=C2)NC(=O)/C=C/C3=CC=C(C=C3)/C=C/C(=O)NC4=CC=C(C=C4)C5=NCCN5.Cl.Cl
  Pathways:Metabolism
  Receptor:neutral sphingomyelinase
  Boling pt:
  Melting pt:
  Solubility:Soluble in DMSO
  Appearance:Powder
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Marchesini, N., et al. 2003. J. Biol. Chem. In press.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting