• Product NameHoechst 33258
• Brief DescriptionInhibitors
• Purification98.00%
• Biological ActivityHoechst 33258 is a cell-permeable, benzimidazole dye that binds to the minor groove of double stranded DNA with preference for adenine and thymine-rich sequences. It emits blue fluorescence (excitation 352 nm/emission maximum 461 nm) when bound to DNA in either live or fixed cells and is useful as a marker of nuclei for cell cycle studies and to distinguish nuclear morphology in apoptotic cells.
• Target NameOthers
• CAS No. 23491-45-4
• Calculated MW 533.9
• Formulation C25H24N6O ? 3HCl
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Others
  SMILES:CN1CCN(C2=CC(N=C(C3=CC(N=C(C4=CC=C(O)C=C4)N5)=C5C=C3)N6)=C6C=C2)CC1.Cl.Cl.Cl
  Pathways:Others
  Receptor:Others
  Boling pt:
  Melting pt:
  Solubility:Water: 20 mM
  Appearance:
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting