• Product NameSAG hydrochloride
• Brief DescriptionInhibitors
• Purification98.00%
• Biological ActivitySmoothened Agonist (SAG) HCl is a cell-permeable Smoothened (Smo) agonist with EC50 of 3 nM in Shh-LIGHT2 cells.
• Target NameHedgehog/Smoothened agonist
• CAS No. S4211
• Calculated MW 526.52
• Formulation C28H29Cl2N3OS
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Others
  SMILES:CNC1CCC(CC1)N(CC2=CC=CC(=C2)C3=CC=NC=C3)C(=O)C4=C(C5=CC=CC=C5S4)Cl.Cl
  Pathways:GPCR/G Protein; Stem Cells
  Receptor:Hedgehog/Smoothened
  Boling pt:
  Melting pt:
  Solubility:Water 100 mg/mL (189.92 mM) DMSO 71 mg/mL warmed (134.84 mM) Ethanol 40 mg/mL warmed (75.97 mM) In vivo Add solvents individually and in order: water 100mg/mL
  Appearance:
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Chen JK, et al. Small molecule modulation of Smoothened activity. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14071-6.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting