• Product NameAutophinib
• Brief DescriptionInhibitors
• Purification98.00%
• Biological ActivityAutophinib is a potent autophagy inhibitor with a novel chemotype with IC50 values of 90 and 40 nM for autophagy in starvation induced autophagy assay and rapamycin induced autophagy assay. The IC50 value for Vps34 is 19 nM in vitro.
• Target NameAutophagy inhibitor; PI3K inhibitor
• CAS No. 1644443-47-9
• Calculated MW 346.731
• Formulation C14H11ClN6O3
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Others
  SMILES:CC1=CC(=NN1)NC2=CC(=NC(=N2)OC3=CC=C(C=C3)[N+](=O)[O-])Cl
  Pathways:Autophagy; PI3K/Akt/mTOR signaling
  Receptor:rapamycin-induced autophagy; starvation-induced autophagy; VPS34
  Boling pt:
  Melting pt:
  Solubility:DMSO 69 mg/mL (199.0 mM) Water Insoluble Ethanol Insoluble
  Appearance:
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Robke L, et al. Phenotypic Identification of a Novel Autophagy Inhibitor Chemotype Targeting Lipid Kinase VPS34. Angew Chem Int Ed Engl. 2017 Jul 3;56(28):8153-8157.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting