• Product NameDNA pol delta cat antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 685 and 1041 of DNA pol delta cat
• Target NameDNA pol delta cat
• Alternative Names    CDC2; POLD
• Accession No. Swiss-Prot:P28340
  Gene ID:5424
• UniprotP28340
• Gene ID5424;
• Concentration 0.6mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 124kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1, POLD2 (MIM 600815), POLD3 (MIM 611415), and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM]

• Sample (30 ug of whole cell lysate)
  A: Molt-4
  7.5% SDS PAGE
  Primary antibody diluted at 1: 3000
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA Polymerase delta, catalytic subunit antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting