Binuclease is a genetically engineered endonuclease from Serratia marcescens gene that can digest all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. Binuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester.
Specific activity: 1000 kU/mg protein
Grade: Benzonase equivalent
Source: From yeast cells with cloned gene encoding genetically engineered Serratia marcescens endonuclease.
E.C.: 3.1.30.2
pH range: pH 6~10, optimum pH 8.0
Temperature profile: Optimum temperature 37°C; Temperature range of enzyme activity: 0~42°C.
Unit Definition: According to the protocol from Sigma-Aldrich, one unit of Binuclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 minutes at pH 8.0 at 37°C.
Advantages: Binuclease is produced from eukaryotic yeast cells that avoid the contamination of endotoxin from prokaryotic cells. Binuclease is highly stable under harsh industrial conditions and very cost effective, which make it a perfect alternative for Benzonase® endonuclease and other equivalents. The specific activity of Binuclease was measured at ≥ 1000 kU/mg protein, which is much higher than any existing products (as much as 200%).
Activators: 1~10 mM Mg2+
Stability: Lyophilized powder is stable for 3 years when stored below 4°C. Buffered aqueous glycerol solution is stable for 1 year when stored at -20°C.
Handling and Storage: Lyophilized powder: shipped at room temperature and storage recommended below 4°C. Buffered aqueous glycerol solution: shipped with blue ice and storage recommended at -20°C. Dilution buffer: 20mM Tris-Cl (pH 8.0), 2mM MgCl2, 20mM NaCl. Storage buffer: 20mM Tris-HCl (pH 8.0), 2mM MgCl2, 20mM NaCl, 50% glycerol.
Precautions and Disclaimer: This product is for R&D use only, not for drug, household, or other uses.
