• Product NamePGK1 (Acetyl-Lys388) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsWB IHC IP
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of PGK1 only when acetylated at Lysine 388.
• Immunogen TypePeptide
• Immunogen DescriptionPeptide sequence around acetylation site of lysine 388(E-D-K(Acetyl)-V-S) derived from Human PGK1.
• Alternative Names    MGC117307,MGC142128,MGC8947,MIG10,PGKA
• Accession No. Swiss-Prot#: P00558
  NCBI Gene ID: 5230
  NCBI mRNA#: NM_000291.3
  NCBI Protein#: NP_000282.1
• UniprotP00558
• Gene ID5230;
• Calculated MW 45KD
• Concentration 1.0mg/mL
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C

•  WB dilution: 1:500~1:1000

   IHC dilution: 1:50~1:100

   IP dilution: 1:20~1:50

• Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D).

Xu Qian1,et al. Phosphoglycerate kinase 1 phosphorylates Beclin1 to induce autophagy.Mol Cell. 2017 March 02; 65(5): 917–931.e6. doi:10.1016/j.molcel.2017.01.027. Autho

• The indicated His-PGK1 proteins immobilized on Ni-NTA agarose beads was incubated with Flag-ARD1 and Ac-CoA, followed by incubation with purified GST-Beclin1 or GST-Beclin1 S30A, in the presence of [γ-32P] ATP. Autoradiography was performed.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting