• Product NameRecombinant Mouse Perforin-1(Prf1)
• Host SpeciesE.coli
• Species ReactivityMs
• Target NamePrf1
• Alternative Names    Short name: P1 Alternative name(s): Cytolysin Lymphocyte pore-forming protein
• Accession No. Uniprot ID: P10820
• UniprotP10820
• Gene ID18646;
• SDS-PAGE MW 63.98kDa
• Target Length Full Length,21-554aa
• Tag Info N-terminal 6xHis-tagged
• Target Sequence PCYTATRSECKQKHKFVPGVWMAGEGMDVTTLRRSGSFPVNTQRFLRPDRTCTLCKNSLMRDATQRLPVAITHWRPHSSHCQRNVAAAKVHSTEGVAREAAANINNDWRVGLDVNPRPEANMRASVAGSHSKVANFAAEKTYQDQYNFNSDTVECRMYSFRLVQKPPLHLDFKKALRALPRNFNSSTEHAYHRLISSYGTHFITAVDLGGRISVLTALRTCQLTLNGLTADEVGDCLNVEAQVSIGAQASVSSEYKACEEKKKQHKMATSFHQTYRERHVEVLGGPLDSTHDLLFGNQATPEQFSTWTASLPSNPGLVDYSLEPLHTLLEEQNPKREALRQAISHYIMSRARWQNCSRPCRSGQHKSSHDSCQCECQDSKVTNQDCCPRQRGLAHLVVSNFRAEHLWGDYTTATDAYLKVFFGGQEFRTGVVWNNNNPRWTDKMDFENVLLSTGGPLRVQVWDADYGWDDDLLGSCDRSPHSGFHEVTCELNHGRVKFSYHAKCLPHLTGGTCLEYAPQGLLGDPPGNRSGAVW
• Formulation Tris-based buffer,50% glycerol
• Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

• Greater than 80% as determined by SDS-PAGE.

 Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.

 "The molecular basis for perforin oligomerization and transmembrane pore assembly."Baran K., Dunstone M., Chia J., Ciccone A., Browne K.A., Clarke C.J.P., Lukoyanova N., Saibil H., Whisstock J.C., Voskoboinik I., Trapani J.A.Immunity 30:684-695(2009)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting