• Product NameC12orf51 Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.A2-F5-F10
• PurificationProA affinity purified
• ApplicationsWB,IHC,ICC,FC
• Species ReactivityHu, Ms
• Immunogen DescriptionRecombinant protein
• Alternative Names    C12orf51 antibody
  HECT domain-containing protein 4 antibody
  HECTD4 antibody
  KIAA0614 antibody
  Probable E3 ubiquitin-protein ligase HECTD4 antibody
  
• Accession No. Swiss-Prot#:Q9Y4D8
• UniprotQ9Y4D8
• Calculated MW 438/145/98/262 kDa
• Formulation 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500
  IHC: 1:50-1:100
  ICC: 1:50
  
  FC: 1:50-1:100

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

• Western blot analysis of C12orf51 on SH-SY5Y (1) and A549 (2) using anti-C12orf51 antibody at 1/100 dilution.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
• ICC staining C12orf51 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining C12orf51 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of SH-SY5Y cells with C12orf51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting