• Product NameFAK(Phospho-Tyr925) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of FAK only when phosphorylated at tyrosine 925.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 925 (K-V-Y(p)-E-N) derived from Human FAK.
• Target NameFAK
• ModificationPhospho
• Alternative Names    FADK 1; FAK1; PTK2
• Accession No. Swiss-Prot: Q05397
  NCBI Protein: NP_005598.3
• UniprotQ05397
• Gene ID5747;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 125kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Sanders MA, et al. (2005) J Biol Chem; 280(25): 23516-22.
Cherubini A, et al. (2005) Mol Biol Cell; 16(6): 2972-83.
Toriumi Y, et al. (2003) FEBS Lett; 553(3): 419-22.

• Western blot analysis of extracts from 293 cells using FAK(Phospho-Tyr925) Antibody #11123(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
• Immunofluorescence staining of methanol-fixed Hela cells using FAK(Phospho-Tyr925) Antibody #11123.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting