• Product NameGAPDH Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.1-G2
• PurificationProA affinity purified
• ApplicationsWB, ICC
• Species ReactivityHu,Ms,Rt,zebrafish
• Immunogen Descriptionpeptide
• Alternative Names    38 kDa BFA-dependent ADP-ribosylation substrate antibody
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  G3PDH antibody
  GAPD antibody
  GAPDH antibody
  Glyceraldehyde 3 phosphate dehydrogenase antibody
  Glyceraldehyde-3-phosphate dehydrogenase antibody
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  KNC-NDS6 antibody
  MGC102544 antibody
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• Accession No. Swiss-Prot#:P04406
• UniprotP04406
• Gene ID2597;
• Calculated MW 36 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:2000-1:5,000
  
  ICC: 1:200
  
  

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

• Western blot analysis of GAPDH on different cells lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: PC-12 cell lysate Lane 5: F9 cell lysate
• ICC staining GAPDH in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
• ICC staining of GAPDH in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor 555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
• Western blot analysis of GAPDH on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting