• Product NameDYKDDDDK Tag(FLAG) Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.A2-A4
• PurificationProG affinity purified
• ApplicationsWB, IP
• Immunogen Descriptionpeptide
• Alternative Names    DDDDK epitope tag antibody
  DDDK antibody
  ddk antibody
  DYKDDDDK antibody
  DYKDDDDK epitope tag antibody
  DYKDDDDK tag antibody
  ECS epitope tag antibody
  ECS tag antibody
  Enterokinase Cleavage Site epitope tag antibody
  Enterokinase Cleavage Site tag antibody
  FLAG antibody
  FLAG tag antibody
  
• Accession No. Swiss-Prot#:
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:5,000-1:10,000
  
  
  IP: 1:10-1:50
  

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

• Western blot analysis on DYKDDDDK Tag recombinant protein (65kDa).
• Immunoprecipitation analysis of different lysates using anti-FLAG antibody. Lane 1: Non-transfected 293T Lane 2: Transfected 293T Lane 3: Agarose beads complexes Lane 4: Negative control (Normal mouse IgG)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting