• Product NameNa+/K+ ATPase Antibody
• Host SpeciesMouse
• Clone No.1G1
• PurificationProA affinity purified
• ApplicationsWB, IP, IF, IHC(P)
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionAmino acids 551-850 of Na+/K+-ATPase α1 of human origin.
• Alternative Names    ATPase Na+/K+ transporting alpha antibody
  adenosinetriphosphatase antibody
  AT1A1_HUMAN antibody
  ATP1A1 antibody
  ATP1A4 antibody
  ATP1AL2 antibody
  ATP1B antibody
  ATP1B1 antibody
  ATPase Na+/K+ transporting alpha 1 polypeptide antibody
  ATPase Na+/K+ transporting alpha 4 polypeptide antibody
  ATPase Na+/K+ transporting beta 1 polypeptide antibody
  ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
  ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
  Beta 1-subunit of Na(+),K(+)-ATPase antibody
  Na(+)/K(+) ATPase alpha-1 subunit antibody
  Na(+)/K(+) ATPase alpha-4 subunit antibody
  Na+, K+ ATPase alpha subunit antibody
  Na+/K+ ATPase 1 antibody
  Na+/K+ ATPase 4 antibody
  Na+/K+ ATPase, alpha-D polypeptide antibody
  Na, K-ATPase beta-1 polypeptide antibody
  Na, K-ATPase, alpha-A catalytic polypeptide antibody
  Na,K-ATPase catalytic subunit alpha-A protein antibody
  Na,K-ATPase subunit alpha-C antibody
  polypeptide-like 2 antibody
  Sodium pump 1 antibody
  sodium pump 4 antibody
  Sodium pump subunit alpha-1 antibody
  sodium pump subunit alpha-4 antibody
  sodium pump subunit beta-1 antibody
  sodium-potassium ATPase catalytic subunit alpha-1 antibody
  sodium-potassium ATPase catalytic subunit alpha-4 antibody
  sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
  sodium-potassium ATPase, alpha 4 polypeptide antibody
  sodium-potassium-ATPase, alpha 1 polypeptide antibody
  Sodium/potassium transporting ATPase alpha 1 chain antibody
  Sodium/potassium transporting ATPase subunit beta 1 antibody
  sodium/potassium-dependent ATPase beta-1 subunit antibody
  sodium/potassium-transporting ATPase alpha-4 chain antibody
  sodium/potassium-transporting ATPase beta-1 chain antibody
  Sodium/potassium-transporting ATPase subunit alpha-1 antibody
  sodium/potassium-transporting ATPase subunit alpha-4 antibody
  
• Accession No. Swiss-Prot#:P05023
• UniprotP05023
• Gene ID476;
• Calculated MW 100-143kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at 4˚C

• WB: 1:2,000-1:1,0000
  IHC: 1:50-1:500
  
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.

• Western blot analysis of Na+/K+-ATPase α expression in human kidney (A) and human brain (B) tissue extracts.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting