• Product NamePCNA Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.A6-G11
• PurificationProA affinity purified
• ApplicationsWB, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    ATLD2 antibody
  cb16 antibody
  Cyclin antibody
  DNA polymerase delta auxiliary protein antibody
  etID36690.10 antibody
  fa28e03 antibody
  fb36g03 antibody
  HGCN8729 antibody
  MGC8367 antibody
  Mutagen-sensitive 209 protein antibody
  OTTHUMP00000030189 antibody
  OTTHUMP00000030190 antibody
  PCNA antibody
  Pcna/cyclin antibody
  PCNA_HUMAN antibody
  PCNAR antibody
  Polymerase delta accessory protein antibody
  Proliferating cell nuclear antigen antibody
  wu:fa28e03 antibody
  wu:fb36g05 antibody
  
• Accession No. Swiss-Prot#:P12004
• UniprotP12004
• Gene ID5111;
• Calculated MW 29 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:200
  
  
  FC:1:100

PCNA (Proliferating cell nuclear antigen) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. It induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities.

• Western blot analysis of PCNA on different cell lysates using anti-PCNA antibody at 1/1000 dilution. Positive control:
  Line 1: L929
  Line 2 :MCF-7
  Line 3:PC12
  Line 4:Raji
  Line 5:F9
  Line 6:A549
• Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PCNA antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-PCNA antibody. Counter stained with hematoxylin.
• Flow cytometric analysis of Hela cells with PCNA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting