• Product NameCD133 Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.D2-B1
• PurificationPeptide affinity purified
• ApplicationsIHC,ICC
• Species ReactivityHu, Ms
• Immunogen DescriptionPeptide
• Alternative Names    AC133 antibody
  Antigen AC133 antibody
  CD133 antibody
  CORD12 antibody
  Hematopoietic stem cell antigen antibody
  hProminin antibody
  MCDR2 antibody
  MSTP061 antibody
  OTTHUMP00000217744 antibody
  OTTHUMP00000217745 antibody
  OTTHUMP00000217746 antibody
  PROM1 antibody
  PROM1_HUMAN antibody
  Prominin I antibody
  Prominin like 1 antibody
  Prominin like protein 1 precursor antibody
  Prominin mouse like 1 antibody
  Prominin-1 antibody
  Prominin-like protein 1 antibody
  Prominin1 antibody
  PROML1 antibody
  RP41 antibody
  STGD4 antibody
  
• Accession No. Swiss-Prot#:O43490
• UniprotO43490
• Gene ID8842;
• Calculated MW Predicted band size 97 kDa
• Formulation 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• 
  IHC: 1:50-1:200
  ICC: 1:100-1:500
  
  

CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. While the precise function of CD133 remains unknown, it has been proposed to act as an organizer of cell membrane topology.

• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD133 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD133 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD133 antibody. Counter stained with hematoxylin.
• ICC staining CD133 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD133 (green) in N2A cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting