• Product NameAnti-Taq DNA Polymerase Antibody
• Host SpeciesMouse
• PurificationProG affinity purified
• ApplicationsAntibody-mediated hot start PCR
• Immunogen DescriptionTaq DNA Polymerase
• Storage Store at -20˚C

Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.

• Application of the hot start PCR using anti-Taq DNA Polymerase antibody
  Lane1: Taq DNA Polymerase
  Lane2: Taq DNA Polymerase + Anti-Taq DNA Polymerase antibody
  Lane3: Taq DNA Polymerase + Control antibody
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting