• Product NameFAN antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 262 and 540 of Human NSMAF
• Target NameFAN
• Alternative Names    FAN
• Accession No. Swiss-Prot:Q92636
  Gene ID:8439
• UniprotQ92636
• Gene ID8439;
• Concentration 0.9mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 104kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:500
  

This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.

• Western Blot analysis of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody.
  A: Non-transfected lysate.
  B: NSMAF transfected lysate.
  7.5% SDS PAGE
  Primary antibody diluted at 1: 500
• Immunohistochemical analysis of paraffin-embedded T(gastric CA), using NSMAF antibody at 1: 100 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting