• Product NameSK2 Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.E1-C5
• PurificationProA affinity purified
• ApplicationsICC, IHC, FC
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein
• Alternative Names    3 prime phosphoadenosine 5 prime phosphosulfate synthase 2 antibody
  3' phosphoadenosine 5' phosphosulfate synthase 2 antibody
  ATP sulfurylase/adenosine 5' phosphosulfate kinase antibody
  ATP SULFURYLASE/ADENOSINE 5-PRIME PHOSPHOSULFATE KINASE 2 antibody
  ATP sulfurylase/APS kinase 2 antibody
  ATPSK2 antibody
  Bifunctional 3' phosphoadenosine 5' phosphosulfate synthase 2 antibody
  Bifunctional 3' phosphoadenosine 5' phosphosulfate synthethase 2 antibody
  PAPS synthase 2 antibody
  PAPS synthetase 2 antibody
  PAPSS 2 antibody
  PHOSPHOADENOSINE-PHOSPHOSULFATE SYNTHETASE 2 antibody
  SK 2 antibody
  SK2 antibody
  Sulfurylase kinase 2 antibody
  
• Accession No. Swiss-Prot#:Q9NRA0
• UniprotQ9NRA0
• Gene ID56848;
• Calculated MW 69 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• 
  IHC: 1:200-1:1000
  ICC: 1:200-1:1000
  
  FC: 1:50-1:100

This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

• Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti- SPHK2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti- SPHK2 antibody. Counter stained with hematoxylin.
• ICC staining SPHK2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with SPHK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting