• Product NameBmi1 Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.B3-G5
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein within human Bmi1 full sequence.
• Alternative Names    B lymphoma Mo MLV insertion region (mouse) antibody
  B lymphoma Mo MLV insertion region 1 homolog antibody
  Bmi 1 antibody
  BMI1 antibody
  BMI1 polycomb ring finger oncogene antibody
  BMI1_HUMAN antibody
  Flvi 2/bmi 1 antibody
  FLVI2/BMI1 antibody
  MGC12685 antibody
  Murine leukemia viral (bmi 1) oncogene homolog antibody
  Oncogene BMI 1 antibody
  PCGF 4 antibody
  PCGF4 antibody
  Polycomb complex protein BMI 1 antibody
  Polycomb complex protein BMI-1 antibody
  Polycomb group protein Bmi1 antibody
  Polycomb group ring finger 4 antibody
  Polycomb group RING finger protein 4 antibody
  RING finger protein 51 antibody
  RNF 51 antibody
  RNF51 antibody
  
• Accession No. Swiss-Prot#:P35226
• UniprotP35226
• Gene ID100532731;648;
• Calculated MW 37kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000
  IHC: 1:200
  ICC: 1:200
  
  FC: 1:100-1:200

The Bmi-1 was identified initially as an oncogene that cooperates with c-myc in the generation of B-cell lymphoma. It contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest.

• Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.
  Positive control:  
  Lane 1: 293T             Lane 2: Jurkat
  Lane 3: Hela              Lane 4: MCF-7
  Lane 5: HepG2           Lane 6: NIH/3T3
  Lane 7: PC12             Lane 8: Mouse kidney
  Lane 9: Human kidney     Lane 10: K562
  Lane 11: Human brain

• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.
• ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting